描述The Amplex® Red Uric Acid/Uricase Assay Kit provides a sensitive and simple method for detecting uric acid or uricase using either a fluorescence microplate reader or fluorometer.? Detect concentrations as low as 100 nM uric acid or 0.2 mU/mL of uricase activity ? Format allows for multiple time point measurements ? Designed for minimal autofluorescence interference Serum uric acid is the end product of purine metabolism in the body tissues and is cleared through the kidneys by glomerular filtration. Most animals can metabolize uric acid to more readily excreted products, but humans lack the necessary enzyme, urate oxidase (uricase), as a result of the presence of two “nonsense mutations” in the human gene for uricase. The Amplex® Red Uric Acid/Uricase Assay Kit provides an ultrasensitive method for detecting uric acid or for monitoring uricase activity. In the assay, uricase catalyzes the conversion of uric acid to allantoin, hydrogen peroxide, and carbon dioxide. In the presence of horseradish peroxidase (HRP), hydrogen peroxide reacts stoichiometrically with the Amplex® Red reagent to generate the red-fluorescent oxidation product resorufin. Because resorufin has absorption and fluorescence emission maxima of approximately 571 nm and 585 nm, respectively, there is little interference from autofluorescence in most biological samples. |
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