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Thermo Scientific Glycogen is a highly purified polysaccharide derived from oysters. It is an inert carrier which significantly increases the recovery of nucleic acids by alcohol precipitation. Glycogen is insoluble in ethanol and forms a precipitate that traps target nucleic acids. During centrifugation, a visible pellet is formed which greatly facilitates handling of the precipitated nucleic acids. Glycogen quantitatively precipitates nucleic acids from diluted solutions with a higher efficiency than that of tRNA, linear polyacrylamide, or sonicated DNA (see References 1-4). We offer two formulations of aqueous glycogen solutions that allow for fast and efficient DNA or RNA recovery: • Glycogen, RNA grade, can be used for both RNA and DNA precipitation (#R0551) • Glycogen, molecular biology grade, recommended for DNA precipitation (#R0561). Highlights • Ideal for high recovery of oligonucleotides (> 8 bases) and low amounts of DNA/RNA (> 20 pg) from diluted solutions • Forms clearly visible pellets • Does not interfere with enzymatic reactions† • Does not interfere with gel electrophoresis of nucleic acids • Does not interfere with spectrophotometrical determination of DNA/RNA concentration, (A260 to 280 measurements) • Compatible with DNA transformation and in vitro transfection Footnote † – At a final concentration up to 8 µg/ µL, Glycogen does not interfere with PCR, DNA sequencing, DNA digestion by endonucleases, ligation, cDNA synthesis, DNA labeling, in vitrotranscription, or bacterial transformation. At a final concentration up to 0.4 µg/ µL, Glycogen does not affect in vitro transfection of eukaryotic cells. |
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