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Thermo Scientific dUTP (2'-deoxyuridine 5'-triphosphate) is highly stable nucleotide, supplied as 100 mM aqueous solution titrated to pH 7.3-7.5 with NaOH. The nucleotide has greater than 99% purity, is free of nuclease activities, human and E. coli DNA. It is designed for many different molecular biology applications. Highlights • Greater than 99% purity—confirmed by HPLC • Free of human and E. coli DNA • Highly stable Application tested in: • Long range PCR (40 kb) • cDNA synthesis and RT-PCR • Real-time PCR • Standard PCR • High fidelity PCR • Applications • For use in all molecular biology applications, including PCR, real-time PCR and RT-PCR with Taq DNA polymerase, cDNA synthesis, and primer extension. Note: dUTP is not suitable for PCR with proofreading enzymes or enzyme mixes containing proofreading enzymes. |
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