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CellROX® Green Reagent is a novel fluorogenic probe for measuring oxidative stress in live cells. The cell-permeant dye is weakly fluorescent while in a reduced state and exhibits bright green photostable fluorescence upon oxidation by reactive oxygen species (ROS) and subsequent binding to DNA, with absorption/emission maxima of ~ 485/520 nm. This reagent can be formaldehyde-fixed and its signal survives detergent treatment, which makes it multiplexable with other compatible dyes and antibodies. CellROX® Green Reagent is compatible with various platforms, such as traditional fluorescence microscopy, high content screening (HCS), flow cytometery, and microplate-based fluorimetry or high throughput screening (HTS). This reagent is also compatible with various benchtop instruments such as our FLoid™, Tali™, and Attune™ instruments. In addition, CellROX™ Green Reagent is: • Compatible with other live cell dyes, antibodies, and in cells expressing RFP • Provided as a stable, ready-to-use DMSO solution with a simple no-wash protocol compatible with standard workflows in fluorescence microscopy • Provided as five single-use vials containing sufficient total reagent for five 96-well plates or 100 coverslips Oxidative stress results from an imbalance between the production of reactive oxygen species (ROS) and the ability of cells to scavenge them. ROS play an important role in the progression of several diseases, including inflammation, atherosclerosis, aging, and age-related degenerative disorders. For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use. |
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