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SYBR® Select Master Mix is a superior and cost-effective solution for your real-time PCR applications. Features of the SYBR® Select Master Mix include: • Specific—minimize primer-dimer and non-specific amplification • Reproducible and sensitive—consistent amplification across a wide dynamic range • Bright—contains SYBR® GreenER™ dye for maximum brightness • Carry-over contamination control—contains heat-labile UDG • Can be used in either standard or fast cycling mode Formulated for Maximum Specificity SYBR® Select Master Mix contains all the components needed for your real-time PCR reaction, except the template and primers, in a convenient 2X concentration premix. The master mix includes AmpliTaq® DNA Polymerase, UP, a highly purified DNA polymerase with a proprietary hot start mechanism that provides exceptional specificity. Obtain Reproducible Results Across a Wide Dynamic Range SYBR Select Master Mix is specially formulated to provide robust results at 100 ng to 0.1 pg cDNA per reaction (Figure 1) and can reliably detect a single copy of genomic DNA (Figure 2). Contains SYBR® GreenER™ Dye and Heat-labile UDG • SYBR® GreenER™ dye is less inhibitory to PCR than SYBR® Green I dye resulting in brighter signals • Heat-labile uracil-DNA glycosylase (UDG) is included for worry-free carryover contamination control Instrument Compatibility SYBR® Select Master Mix can be used in either standard or fast cycling mode and is compatible with all Applied Biosystems® real-time PCR instruments except the 7900HT real-time PCR system. It is also compatible with the Bio-Rad IQ™5, Roche LightCycler® LC480, and Stratagene® MX3005P® systems. View performance data. Alternative product Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of SYBR Select Master Mix and added additional capabilities for your gene expression analysis. |
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