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Alexa Fluor® 647 Carboxylic Acid is a non-reactive form of Alexa Fluor® 647 that can be used as a reference standard for dye-conjugates. Additionally, this carboxylic acid derivative can be converted to an amine-reactive ester using standard chemical techniques or coupled to hydrazines, hydroxylamines, and amines in aqueous solution using water-soluble carbodiimides such as EDAC (Cat. No. E2247).
Characteristics of Alexa Fluor® 647 dye include: • Laser line: 647 nm • Common filter set: Cy® 5 • Excitation maxima: 650 nm • Emission maxima: 665 nm More About Alexa Fluor® 647 Dye Alexa Fluor® 647 dye is a bright, far-red-fluorescent dye with excitation ideally suited for the 594 nm or 633 nm laser lines. It is ideal for imaging low-abundance targets, especially in fixed cells. Alexa Fluor® 647 dye is commonly used with DAPI, and Alexa Fluor® 488 and 594 dyes for multiplexing. Learn more about this dye and related products. Discover the Benefits of the Alexa Fluor® Dye Series Compared to traditional dyes, Alexa Fluor® dyes are brighter, more photostable, and more pH-resistant between pH 4 and 10. And generally when using Alexa Fluor® dyes, higher degrees of labeling can be achieved without intramolecular quenching. For details see Alexa Fluor® Dyes Spanning the Visible and Infrared Spectrum in the Molecular Probes Handbook. |
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