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New pH-sensitive pHrodo® dye conjugates give faster and more accurate results than any other phagocytosis assay. pHrodo® dye conjugates are non-fluorescent outside the cell, but fluoresce brightly red in phagosomes. Get faster staining and more accurate results—without the need for wash steps or quencher dye. • Specific detection of phagocytosis and endocytosis • Reduced signal variability and improved timing in sensitive experiments. • Multiplex with green dyes such as GFP, Fluo-4, or calcein The fluorescence of the novel pHrodo® dye dramatically increases as pH decreases from neutral to acidic, making it an ideal tool to study phagocytosis and its regulation by drugs and/or environmental factors. The lack of fluorescence outside the cell eliminates the need for wash steps and quencher dyes. Use the ready-made pHrodo® E.coli BioParticles® conjugates in imaging or flow applications, or pHrodo® SE, the activated succinimidyl ester, for labeling microorganisms or proteins of your choice. |
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