描述Dynabeads® Mouse T-Activator CD3/CD28 is for the activation and expansion of mouse T-cells for research purposes.With Dynabeads® Mouse T-Activator CD3/CD28 you get: • Activation of T-cells without the need of feeder cells • Activated cells that retain in vivo-like function • Maximal reproducibility without contamination by soluble antibodies or mitogens • From 100- to 1000-fold T-cell expansion How Dynabeads® Mouse T-Activator CD3/CD28 works: Dynabeads® Mouse T-Activator CD3/CD28 offers a simple method for activation and expansion of T-cells that does not require feeder cells (antigen-presenting cells) or antigen. The 4.5 µm diameter, inert, superparamagnetic beads are similar in size to antigen-presenting cells, and are covalently coupled to anti-CD3 and anti-CD28 antibodies. These two antibodies provide primary and co-stimulatory signals, optimized for efficient T-cell activation and expansion. Expansion of the T-cell population can be stimulated using recombinant IL-2, and after activation or expansion, the magnetic beads can be easily removed using a DynaMag™ magnet. For expansion of antigen specific T-cells from T-cell clones or T-cell lines, we recommend using Dynabeads® Mouse T-Activator CD3/CD28/CD137. Starting samples Start with single cell suspensions from spleen or lymph nodes, mononuclear cells (MNCs), whole blood, or T-cell subsets including CD3+ T cells, CD4+ or CD8+ T cells, or regulatory T cells. Get an overview of the different Dynabeads® available for cell isolation. Downstream applications The activated T-cells can be analyzed shortly after activation (for transfection/transduction or to study TCR signaling, proteomics, gene expression, etc.). T-cells can be left in culture to differentiate into T helper cell subsets or expansion of polyclonal/antigen-specific T cells.
For Research Use Only. Not for use in diagnostic procedures.
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